Russia have eliminated Spain from the World Cup in a 4-3 penalty shootout, the hosts winning their last‑16 tie after the sides were locked 1-1 after extra time at the Luzhniki Stadium in Moscow.
An own goal from the Russia defender Sergei Ignashevich gave Spain an early lead. Artem Dzyuba then converted a penalty three minutes before the break to pull the tournament hosts level at half-time.
Their quarter-final opponents will be decided on Sunday night, when Croatia face Denmark in Nizhny Novgorod.
Fifa approved the rule in March for an additional substitute in extra time at the World Cup, but had earlier used it at youth tournaments and the 2016 Olympic football tournament
The teams could not be separated after 90 and then 120 minutes, signalling the first penalty shoot-out of the 2018 World Cup.
Iago Aspas and Koke both missed from 12 yards, with the shoot-out ending 4-3.
Spain now join Germany, Argentina and Portugal as the fancied teams eliminated.